|
System
|
Software for the rapid analysis of 2D protein spots between samples containing replicate gels, stained with visible or fluorescent dyes, and the analysis of multi-stained fluorescent gels
|
|
On screen views
|
Main pane and secondary panes for viewing replicates and samples. Syncronised zoom with intensity autoscale
|
|
Image processing
|
Automatic and precise image warping with vector view. Manual warp editing. Automatic background subtraction and noise filtering. SYPRO® Ruby Crystal filter
|
|
Spot detection
|
Automatic spot detection. Region of interest option. Gaussian spot modelling option for non-multiplexed analysis
|
|
Speed of spot detection
|
3x10 Mbyte images in eight seconds
|
|
Automatic spot matching
|
Generates averaged gel image and performs average gel warping. Matches spots
|
|
Spot editing
|
Spot detection with user adjustable parameters. Spot editing, filtering and normalisation
|
|
Image calibration
|
pl and MW ladder calibration. Protein list assignment of spots with known identity, pl and MW
|
|
Data visualisation
|
Interactive process flow diagram. Histogram (brightness and contrast). Main pane and secondary panes for multi-stained gel sets, replicates and samples. Bar chart. 3D spot profile. Table of spot matching results. Scatter plot. Correlation plot.
|
