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System
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Software for the rapid analysis of 2D protein spots between samples containing replicate gels stained with visible or fluorescent dyes
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On screen views
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Main pane and secondary panes for viewing replicates and samples. Syncronised zoom with intensity autoscale
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Image processing
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Automatic and precise image warping with vector view. Manual warp editing. Automatic background subtraction and noise filtering. SYPRO® Ruby Crystal filter
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Spot detection
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Automatic spot detection. Region of interest option. Gaussian spot modelling option
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Speed of spot detection
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3x10 Mbyte images in eight seconds
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Automatic spot matching
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Automatic warping and spot matching across multiple samples
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User definable parameters
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Spot detection with user adjustable parameters. Spot editing, filtering and normalisation
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Image calibration
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pl and MW ladder calibration. Protein list assignment of spots with known identity, pl and MW
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Data visualisation
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Interactive process flow diagram. Histogram (brightness and contrast). Main pane and secondary panes for replicates and samples. Bar chart. 3D spot profile. Table of spot matching results. Scatter plot. Correlation plot.
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